Indices of genetic diversity of HAV sequences (VP1-2A region) from Lazio region, grouped according to the year of sampling (2013–2015 2016, 2017, 2018).

N: sample size; K n. of variant sequences; S: n. of polymorphic sites; H: gene diversity; Tajima's D:test to highlight in population any deviation from neutral evolution; dN/dS: ratio of non-synonymous mutations per non-synonymous site (dN) and synonymous mutations per synonymous site (dS) of all viral sequences from each viral population in years.