Replication data for Gap junction mediated glycinergic inhibition ensures precise temporal patterning in vocal behavior

The data files are all in Axon instruments format How to open files: Visit axon instruments to download their software. Alternatively use IGOR (Wavemetrics) and install the neuromatic software ( http://www.neuromatic.thinkrandom.com/). The vocal nerve recordings are extracellular recordings- stimulus artefact is induced either by current stimulation in the midbrain, or by implanted electrodes in the muscle. Data is composed of intracellular recordings in current clamp (i.e. Voltage changes are recorded, this is not always apparent in the axon files, ignore the labeling of the y axis). A pulse was given at the beginning of each trace by the intracellular amplifier as a way to measure the amplitude of the voltage changes. In case the RMP has an offset, this was removed afterwards (the electrode was retracted, and the offset was noted). Current that was applied to the recording electrode is also recorded. The conversion from Voltage change to current readout was done after recording, so the value you are seeing are the voltage changes given out by the amplifier. The midbrain stimulation pulse commands are in another channel (in some of the recordings), in others, it is obvious (stimulation artefact) In the following the subfolder contain the original data files, from which the data was extracted. The XLS files contains the values that were extracted Note: Look at the tree organization of the data. Foldername code:OB-xX-Yy-cxxx OB = Opsanus beta xX = year data was acquired Yy = fish ID -running number cxxx = cell number所有数据文件均采用Axon仪器（Axon Instruments）格式。文件打开方式如下：可访问Axon仪器官网下载其官方配套软件；亦可使用IGOR（Wavemetrics公司出品）并安装neuromatic软件（下载地址：http://www.neuromatic.thinkrandom.com/）。本次收录的喉神经记录为细胞外记录——刺激伪迹可通过中脑电流刺激，或肌肉植入电极两种方式诱导产生。数据集包含电流钳模式下的细胞内记录（即实际记录电压变化，该信息在部分Axon格式文件中并不直观，可直接忽略y轴标注）。每条记录轨迹的起始处均由细胞内放大器施加一个测试脉冲，用于测算电压变化的幅值。若静息膜电位（Resting Membrane Potential，RMP）存在偏移，将在后续处理中予以校正移除（具体操作为缩回记录电极并记录偏移量）。施加于记录电极的电流同样会被同步记录。实验结束后会将原始电压变化转换为电流读数，因此您当前所见的数值实为放大器输出的原始电压变化量。中脑刺激脉冲指令存储于单独通道（部分记录中可直接识别），其余记录中则可通过刺激伪迹快速定位该通道。如下的子文件夹包含用于提取数据的原始文件，所有提取得到的数值均存储于XLS文件中。注意事项：请留意数据集的树形目录组织结构。文件夹命名规则为：OB-xX-Yy-cxxx。其中：OB代表Opsanus beta；xX为数据采集年份；Yy为鱼只流水编号；cxxx为细胞编号。