Plant sample from SC-CO2 garlic extract treated Triticum aestivum

The biostimulant phytochemicals as alternatives to synthetic chemicals are gaining ground in sustainable agricultural production nowadays. The medicinal herb, garlic (Allium sativum) has a spectacular therapeutic reputation due to its antimicrobial properties. The effectiveness of supercritical carbon dioxide (SC-CO2) extraction of A. sativum could help preserve bioactive compounds and be used as a biostimulant agent. The SC-CO2 garlic was formulated in liposomes and used as a nanoscale drug delivery system to reach better efficiency of penetration and translocation. The SC-CO2 garlic extracts were used in Triticum aestivum time-course experiments to monitor conditioning effects such as improving crop quality and priming its defense responses against different pathogens. Fresh leaves were collected after SC-CO2 garlic exposure at 15 min, 24, and 48 hours for QuantSeq 3′ mRNA sequencing at Illumina NextSeq 550 platform