Generalization of negative memories drives the development of psychological distress-related behaviors

To investigate the molecular mechanism of unpredictable aversive stress induced commobidity between fear generalization and depression, mice were treated by different training paterns (1xTr, 2xTr-same and 2xTr-altered). Therefore, mPFC were dissected to perform immunoprecipitation and RNA seq. Overall design: Translating profiles of the BNST-projecting mPFC neurons and transcriptomic profiles of the mPFC in mice after 1xTr, 2xTr-same and 2xTr-altered CFC trainings. Comparative gene expression profiling analysis of RNA-seq data for BNST-projecting mPFC neurons or mPFC tissues under different treatment (1xTr, 2xTr-same, 2xTr-altered). Immunoprecipitation was performed using GFP Recombinant Rabbit Monoclonal Antibody (Invitrogen G10362, Lot2306774).