Bovine vaginal metagenome with mock samples

In addition to the host tissue collected during the sampling process, bovine vaginal samples are saturated with high amounts of extracellular DNA and secreted proteins. Due to the high host-to-microbe genome ratio, which hampers the sequencing efficacy for metagenome samples and the recovery of the actual metagenomics profiles, bovine vaginal samples cannot benefit from the full potential of shotgun sequencing. These are the samples prepared for the first investigation on the most effective host depletion and extraction methods for bovine vaginal metagenomics samples. This study demonstrated an effective combination of host depletion and extraction methods which harvested higher percentages of 16S rDNA and microbial reads, which subsequently led to a taxonomical profile that resembled the actual community and a functional profile with deeper coverage.