R1_Experiment_2Cohort2_Code from Waking inactivity as a welfare indicator in laboratory mice: investigating postures, facial expressions and depression-like states

Animal welfare assessment relies on valid and practical indicators of affect. In mice, the most widely used research vertebrates, lying still with eyes open, inactive-but-awake (IBA) in the home cage, have potential to be one such indicator. IBA is elevated in barren, conventional housing compared to well-resourced, enriched housing and predicts immobility in Forced Swim Tests, a common measure of ‘helplessness’ in depression research. In Experiment 1, using females from three strains (C57BL/6, Balb/c and DBA/2), we fist replicated past findings, confirming higher levels of IBA in conventional cages and a positive relationship between IBA and helplessness. We then extended this research to three other signs of depression: changes in weight and sleep; and reduced hippocampal volume. Here, IBA positively covaried with body mass index, with sleep in DBA/2s and conventionally housed BALB/cs, and negatively covaried with hippocampal volume in conventionally housed C57BL/6s. In Experiment 2, we sought to refine the phenotype of IBA to improve its accuracy as a welfare indicator. Here, IBA performed in hunched postures appeared to improve its accuracy in Balb/c mice. Additional research is now needed to further refine the phenotype of IBA and to confirm whether it reflects states consistent with depression, or instead other underlying conditions.