HBx interacts with USP37 and promotes USP37 translocation from Nucleus to cytoplasm.

Representative confocal image of mock-treated Huh7 cells transiently expressing Vector, X0-GFP or XO-NESM-GFP constructs and cells expressing X0-GFP construct treated with Leptomycin B (100 nM, 4 h), fixed and stained with anti-HBx and anti-USP37 primary antibody and corresponding fluorescent-labelled secondary antibody. Nuclei were counterstained with DAPI. Images were captured at 60x magnification in Nikon A1R confocal microscope. Scale bar represents 10 µm unless mentioned otherwise. (B) Cytoplasmic-nuclear fractionation was performed with cells transfected with vector, HBx-GFP or HBx-NESM-GFP constructs as per the protocol mentioned earlier. The Nuclear (N) and the cytoplasmic (C) fractions of cells were western blotted with USP37, HBx, Histone H1 and GAPDH antibodies. (C) USP37 was immunoprecipitated from cell lysate of Huh7 cells transfected with Vector or HA-HBx. Immuno-complexes were separated on SDS-PAGE gel and were immunoblotted using USP37 and HBx anitibody. Input was probed with HA antibody.