Neurofibromin (Nf1) controls metabolic balance and Notch-dependent quiescence of murine juvenile myogenic progenitors [ChIP-seq]

We conclude that Nf1Myf5 MPs are driven towards quiescence and show metabolic reprogramming with severely inhibited glycolytic metabolism that, in turn impinges on H4K16ac decoration and expression of myogenic differentiation-related genes. ChIP-Seq analysis of freshly isolated p7 MPs showed apparently unaltered H3K4me3 levels between controls and Nf1Myf5 MPs, while H3K27me3 levels were globally reduced in Nf1Myf5 MPs. A reduction of H3K27me3 decoration was observed at genes associated with quiescence as Pax7, or with cell cycle repression as Cdkn2a. The assumption of a quiescent state in Nf1Myf5 MPs is concomitant to decreased repressive H3K27me3 modification at quiescence-associated genes. Three histone modification marks (Histone 3 Lysine 4 trimethylation, Histone 3 Lysine27 trimethylation and Histone 4 Lysine16 acetylation) status were compared through ChIP-Seq analysis between freshly FACS sorted p7 controls and Nf1Myf5 MPs.