Poster Filip Humenik fin.pdf

Adult stem cells have become prominent candidates for treating various diseases in veterinary practice. The main goal of our study was therefore to provide a comprehensive study of canine bone marrow-derived mesenchymal stem cells (BMMSC) and conditioned media, isolated from healthy adult dogs of different breeds. Under well-defined standardized isolation protocols the multipotent differentiation and specific surface markers of MSCs were supplemented with their gene expression, proteomic profile and their biological function. The presented data confirm that canine BMMSC express important genes for differentiation toward osteo-, chondro- and tendo-genic directions, but also genes associated with angiogenic, neurotrophic and immunomodulatory properties. Furthermore, using proteome profiling we identify for the first time the dynamic release of various bioactive molecules, such as transcription and translation factors, osteogenic, growth, angiogenic and neurotrophic factors from canine BMMSC conditioned medium. Importantly, the relevant genes were linked to their proteins as detected in the conditioned medium, and further associated with angiogenic activity in chorioallantoic membrane (CAM) assay. In this way we show that the canine BMMSC release a variety of bioactive molecules revealing a strong paracrine component which may possess therapeutic potential in various pathologies. However, extensive experimental or pre-clinical trials testing canine sources need to be performed in order to better understand their paracrine action, which may lead to novel therapeutic strategies in veterinary medicine.