mRNA sequencing of breast cancer brain metastasis tumors FACS sorted for E-cadherin high and low levels

In this study we want to compare the expression profiles of E-cadherin high and low tumor cells from 4 different mice with breast cancer brain metastasis. We used a breast cancer brain metastasis model derived from the PyMT E-cadherin-mCFP breast cancer model (Beerling et al. Cell Reports 2016) and FACS sorted samples based on endogenous E-cadherin-mCFP expression. Overall design: Examination of expression profiles of E-cadherin high and Ecadherin low tumor cells from 4 mice. Per mouse we FACS sorted a population of E-cadherin high and e-cadherin low tumor cells.