Genome-wide analysis of human endothelium response to Staphylococcus aureus blood isolates infection.

Analysis of strain-specific HUVEC gene expression as a response to infection by selected sepsis isolates of Staphylococcus aureus. Previous study showed great heterogeneity of endothelium pro-inflammatory response, cell death induction and translocation of bacteria through the endothelium barrier. The focus of this study was to investigate different endothelial gene expression patterns which might correspond to activation of different pathways determining various behaviour of the endothelium to the infection. Results give important information on strain-specific endothelium gene response to Staphylococcus aureus infection and give hints about important key players involved in strain-specific endothelium responses. Total RNA obtained from human umbilical cord endothelial cells infected with respective Staphylococcus aureus strain. Expression of infected endothelial cells were compared to mock infection control and tumor necrosis factor alpha treatment control.