Mechanistic Study of Inulin in Ameliorating Heat Stroke-Induced Intestinal Barrier Damage via Modulation of Gut Microbiota- Butyric Acid- GPR43 Axis

Four groups were randomly divided: the control group, inulin group (INU), heat stroke group (HS), and inulin pretreatment plus heat stroke group. When treated with 5% inulin dissolved in drinking water, mice of the INU and INU plus HS groups received standard drinking water indistinguishable to the one fed to Control as well as HS animals slaughtered simultaneously. This inulin intervention took place over 4 weeks. Fecal samples were collected from mice after heat stress and immediately stored at ultra-low temperature refrigerator for further microbiota analysis.