Antibacterial Albumin-Tannic Acid Coatings for Scaffold-Guided Breast Reconstruction

The dataset contains the surface characterisation of 3D-printed non-porous and micro-porous medical-grade polycaprolactone (mPCL) scaffolds via Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and microcomputed tomography. In addition, the mechanical properties of these scaffolds were tested by applying uniaxial compression using an Instron model 5848. 

Human serum albumin (HSA) and tannic acid (TA) were immobilised onto the surface of micro-porous mPCL scaffolds. Herein, we include the surface characterisation of the modified surfaces using FTIR, XPS, and fluorescein 5(6)-isothiocyanate (FITC)-labelling. Finally, the antibacterial in vitro efficacy of HSA/TA-coated scaffolds was assessed in a 2D zone of inhibition assay against S. aureus and P. aeruginosa. Nevertheless, since 2D zone of inhibition assays do not fully represent the interactions of coated scaffolds with bacteria in a realistic in vivo environment, a 3D in vitro assay was designed in order to investigate the antibacterial properties of coated scaffolds against S. aureus in suspension.

The data is available under the 'Access the data' link, as well as in the Supplementary Material section of the article 'Antibacterial Albumin-Tannic Acid Coatings for Scaffold-Guided Breast Reconstruction'.

本数据集收录了通过傅里叶变换红外光谱（Fourier transform infrared spectroscopy, FTIR）、X射线光电子能谱（X-ray photoelectron spectroscopy, XPS）、扫描电子显微镜（scanning electron microscopy, SEM）及显微计算机断层扫描，对3D打印的无孔与微孔医用级聚己内酯（polycaprolactone, mPCL）支架开展的表面表征数据。此外，采用英斯特朗5848型号试验机（Instron model 5848）对上述支架进行单轴压缩测试，以获取其力学性能数据。

将人血清白蛋白（human serum albumin, HSA）与单宁酸（tannic acid, TA）固定于微孔mPCL支架表面。本数据集还包含采用FTIR、XPS及荧光素5(6)-异硫氰酸酯（fluorescein 5(6)-isothiocyanate, FITC）标记法对改性支架表面进行表征所得的数据。最后，通过二维抑菌圈试验，评估了HSA/TA涂层支架对金黄色葡萄球菌（Staphylococcus aureus, S. aureus）与铜绿假单胞菌（Pseudomonas aeruginosa, P. aeruginosa）的体外抗菌活性。

但由于二维抑菌圈试验无法完全反映涂层支架在真实体内环境中与细菌的相互作用，本研究额外设计了三维体外试验，以探究涂层支架对悬浮态金黄色葡萄球菌的抗菌性能。

数据集可通过‘获取数据’链接获取，同时也可在论文《用于支架引导型乳房重建的抗菌白蛋白-单宁酸涂层》（Antibacterial Albumin-Tannic Acid Coatings for Scaffold-Guided Breast Reconstruction）的补充材料部分查阅。