Azithromycin promotes relapse by disrupting immune and metabolic networks after allogeneic stem cell transplantation. Azithromycin promotes relapse by disrupting immune and metabolic networks after allogeneic stem cell transplantation

The aim of this experiment was to identify gene expression changes in sorted T cells from healthy donors two days after CD3/CD28 activation in the presence of azithromycin or a control. Following cell clustering according to their phenotype, gene expression was compared between azithromycin treated and non-treated activated T cells. We identified a downregulation in mitochondrial genes expression and pro-inflammatory genes and upregulation of anti-inflammatory genes such as SOCS1. Overall design: Sorted T cells from human healthy donors, untreated and unactivated, activated with azithromycin, activated and untreated mRNA profiles after two days of CD3/CD28 activation