Expression data from adult transgenic zebrafish

Hepatocellular carcinoma is the fourth leading cause of cancer-related deaths worldwide. Many carcinogens induce inflammation and cirrhosis, and eventually develop into liver cancer. Fucoidan is sulfated polysaccharide that is mainly found in brown seaweeds. In this study, we investigated the effects and mechanisms of low molecular weight fucoidan (i.e. oligo-fucoidan) preventing hepatocarcinogenesis using HBx,Src, and HBx,Src,p53-/+ transgenic zebrafish liver cancer model. Using an oligo-fucoidan oral gavage in adult transgenic zebrafish, we then used microarrays to detail the global programme of gene expression after fucoidan treatment and identified distinct classes of up- and down-regulated genes during this process. Three-month-old female HBx,Src and HBx,Src,p53–/+ transgenic zebrafish were allocated to 2 different diets: normal diet (NOR) and diet-induced obesity (DIO). The amount of Artemia fed to the NOR and DIO groups was approximately 6.9 mg and 83 mg cysts/fish/day respectively. OF (300 mg/kg) was administrated by oral gavage for one month starting at 4 months of age, totally 2 months dietary induction. The fish were sacrificed and the hepatic tissue was immediately frozen in liquid nitrogen and stored at −80 °C for later RNA extraction and hybridization on Affymetrix microarrays.