3-D rendering of renal vasculatures and tubules of rats kidney.mp4

Serial optical sections (z-stack; 230 μm steps
obtained at 1 μm intervals) were generated using an SP8 multiphoton microscope.
A 3-D reconstruction was generated from a
z-stack using Imaris, version 9.2.1. The video frame rate was 20 frames/second.

本研究采用SP8多光子显微镜（SP8 multiphoton microscope）采集得到连续光学切片（z轴堆叠成像（z-stack）；以1 μm为间隔，步长为230 μm）。随后，利用Imaris 9.2.1版本（Imaris, version 9.2.1）对该z轴堆叠成像数据进行三维重建。本次制作的视频帧率为20帧/秒。