Anti-lubricin monoclonal antibodies created using lubricin-knockout mice immunodetect lubricin in several species and in patients with healthy and diseased joints.
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The epitope motif is repeated is present about 20 times in the lubricin protein sequence. The mAb 9g3 more weakly recognized variant peptide forms of the epitope, expressed as a fusion protein with a human IgG-Fc domain, on western blots. These included KEPAATTT, KEPAATT, PAATTT, and KEPAPTAT. MAbs 7h12, 6a8, and 5c11 did not react with peptides tested other than the epitope.
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2015-01-01



