Novel Targets for Diabetic Foot Ulcer Healing: A Transcriptomic Analysis by Negative Pressure Wound Therapy

The results of the present study provide new insights into the mechanism through which NPWT promotes DFU wound healing Overall design: Participants underwent wound debridement to remove blackened and necrotic soft and bone tissues, followed by NPWT. For NPWT, a vacuum assisted closure (VAC) negative pressure-assisted healing therapy system (KCI, USA) was used according to published protocols , at a dose of -125 mmHg for 1 week. Full-thickness skin tissue was taken within 0.5 cm of the wound edge before and after 1 week of NPWT, only after removing the negative pressure device. The collected granulation tissue was stored at -80ºC for examination.RNA-seq was performed on the granulation tissue of three patients with DFU before and after NPWT (before/after-patient 1, before/after-patient 2, before/after-patient 3)