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Immune landscape of mouse heart in steady state

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To reveal the cardiac immune landscape of mouse heart in steady state, we performed single-cell RNA-sequencing (scRNA-seq) with heart tissue from wild type mice and immune subsets were analyzed to reveal cardiac immune features. We performed single-cell RNA-sequencing (scRNA-seq) with heart tissue from wild type C57BL/6 mice (n=6). We constructed one library with CD45+DAPI- cells purified from pooled enzymatically digested hearts by FACS.

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