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Amplicon sequencing for analysis of Cas9-VLP-mediated genome editing. Homo sapiens

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NIAID Data Ecosystem2026-03-12 收录
下载链接:
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA680046
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资源简介:
Virus-like particles (VLPs) have been engineered to package Cas9 protein or pre-assembled Cas9-guide RNA ribonucleoprotein complexes for the purposes of genome editing in mammalian cells. Lentivirus packaging Cas9 (Cas9-VLPs) were produced and use to treat the indicated cell lines. DNA was isolated 3 days post treatment, and targeted genomic regions were PCR amplified, and subjected to deep sequencing (Illumina MiSeq).
创建时间:
2020-11-22
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