Supplementary information: Analysis of natural variation in photosynthesis in a panel of Brassicaceae species

This data supports the conclusions derived from an high- and low-throughput investigation of natural variation in photosynthetic light-use efficiency (LUE), and a number of traits potentially correlated to it, in a panel of ten Brassicaceae species. In this study, I performed an analysis of photosynthetic efficiency at high irradiance in ten species that reflect key evolutionary events within the Brassicaceae family: <em style="background-color:rgb(255, 250, 234);">Arabidopsis thaliana</em>, <em style="background-color:rgb(255, 250, 234);">Brassica oleracea</em>,<em style="background-color:rgb(255, 250, 234);"> Brassica nigra</em>, <em style="background-color:rgb(255, 250, 234);">Brassica rapa</em>, <em style="background-color:rgb(255, 250, 234);">Brassica tournefortii</em>, <em style="background-color:rgb(255, 250, 234);">Erucastrum littoreum</em>, <em style="background-color:rgb(255, 250, 234);">Hirschfeldia incana</em>, <em style="background-color:rgb(255, 250, 234);">Sinapis alba</em>, <em style="background-color:rgb(255, 250, 234);">Sisymbrium irio</em>, and <em style="background-color:rgb(255, 250, 234);">Zahora ait-atta</em>. I made use of high-throughput phenotyping techniques to measure photosynthetic efficiency, and integrated these measurements with other image-based parameters, such as the Excess Green Index (ExGI) and the Normalized Difference Vegetation Index (NDVI), as well as a range of anatomical and biochemical characteristics that potentially influence photosynthetic efficiency. I then explored the resulting multivariate dataset using various statistical methods to identify trends across species and investigated if more species within the Brassicaceae family show high-photosynthetic LUE at high irradiance. Furthermore, I assessed the alignment of these trends with the evolutionary history of the Brassicaceae family. This study delivers a detailed description of inter-specific variation in photosynthetic parameters for the Brassicaceae family, completed by a selection of anatomical and biochemical characteristics that may play a role in supporting high photosynthetic LUE under high irradiance. The gained insights will be important in developing strategies to enhance the photosynthetic LUE at high irradiance of crop species.

本数据集支撑了一项针对十字花科（Brassicaceae）10个物种研究群体的光合光利用效率（photosynthetic light-use efficiency, LUE）自然变异及其若干潜在相关性状开展的高低通量研究所得出的结论。本研究针对体现十字花科家族关键演化事件的10个物种开展了高光强条件下的光合效率分析，这些物种分别为：拟南芥（Arabidopsis thaliana）、甘蓝（Brassica oleracea）、黑芥（Brassica nigra）、白菜（Brassica rapa）、图尔诺夫芥（Brassica tournefortii）、滨海芸芥（Erucastrum littoreum）、赫希菲尔德芥（Hirschfeldia incana）、白芥（Sinapis alba）、筒果糖芥（Sisymbrium irio）以及Zahora ait-atta。本研究采用高通量表型分析技术测定光合效率，并将这些测定结果与其他基于图像的参数相结合，包括过量绿色指数（Excess Green Index, ExGI）和归一化差异植被指数（Normalized Difference Vegetation Index, NDVI），以及一系列可能影响光合效率的解剖学与生化特征。随后，本研究通过多种统计方法对所得多变量数据集展开分析，以识别物种间的演化趋势，并探究十字花科家族内是否存在更多物种在高光强下表现出高光合光利用效率。此外，本研究还评估了这些趋势与十字花科家族演化历史的契合程度。本研究详细描述了十字花科物种间光合参数的种间变异，并补充了一系列可能在高光强下支撑高光合光利用效率的解剖学与生化特征相关数据。本研究所得的见解，将为提升作物物种在高光强下的光合光利用效率的策略开发提供重要参考。