SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in July 2011

Flow cytometry data was collected in July 2011, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (FM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

本数据集的流式细胞术(Flow Cytometry)数据采集于2011年7月，采集区域为澳大利亚南部近岸海域。本研究的总体目标为：建立澳大利亚南部大陆架海域生态系统的背景知识库，明确上升流/盐度异常流出事件对微生物群落的影响，最终构建该区域的生物地球化学模型。采样工作依托澳大利亚南部综合海洋系统(Southern Australian Integrated Marine System, SAIMOS)项目，在RV Ngerin号科考船执行的多个航次中完成。各航次均针对叶绿素荧光最大值(Chlorophyll Fluorescence Maximum, FM)层的物理、化学与生物学特性开展调查。本研究已针对微微型浮游植物、细菌与病毒采集流式细胞术数据。本研究共布设6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）与SAM5CB/B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），位于袋鼠岛西南海域。需说明的是：结合站位间距（14~25海里）、陆架中层海流平均流速分量（0.01 m·s⁻¹）与科考船平均航速（9节），可判定各站位所采集的水样分属不同水团。此外，研究团队还偶尔采集了袋鼠岛国家参考站(National Reference Station, NRS，35.832°S，136.447°E)以及南澳斯宾塞湾口锚系站(SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E，该区域为盐度异常流出事件发生地)的额外样品。