Investigating the expression signatures of circRNA in pulmonary arteial hypertension

To investigate the underlying mechanism of pulmonary hypertension, the model of hypoxia-treated pulmonary arterial hypertension (PAH) rats were constructed to detect the differentially expressed profile of circRNAs in lung tissue of PAH rat. The whole genome microarray expression profiling analysis as a discovery platform have been employed to identify genes difference. The male rats were exposed to hypoxia (10% oxygen) in a ventilated chamber to induce pulmonary arterial hypertension model, and control rats were exposed to normoxic condition. After 3 weeks late, the lung tissue was collected to detect the circRNA expression profile.