Pairwise colocalization of EhARPC1 with EhC2PK, EhCaBP1, EhCaBP3 and EhAK1 during phagocytosis of RBC.

(A)E. histolytica cells were incubated with RBC for 5 min at 37°C. The cells were then fixed and immunostained with anti-EhARPC1 antibody followed by Pacific blue-410. F-actin was stained with TRITC phalloidin and other indicated proteins were immunostained with respective antibodies, followed by Alexa 488-labelled secondary antibody. Arrowhead indicate phagocytic cups, asterisk mark just closed cups before scission and star denotes phagosomes. Scale Bar represents 10 μm. (B) Colocalization analysis from 10 cells was done by using Olympus Fluoview FV1000 software. The Pearson’s coefficient (r) values of EhARPC1 with EhAK1, EhCaBP1, EhCaBP3 and EhC2PK from phagocytic cups are indicated. (C) Schematic representation of the different stages of phagocytosis and the localization of molecules (described in A) during these stages is summarized.