Global gene expression pattern in human first trimester primary villous cytotrophoblast cells (vCTBs) in comparison with human first trimester placental villous mesenchymal cells

vCTBs and mesenchymal cells were isolated from palcental villi of medically terminated and deidentified first trimester placenta (pulled samples from 4-6 placentas of 8-10 week gestation). Overall design: Proliferating vCTBs and mesenchymal cells were isolated through enzymatic digestion followed by a percoll gradient separation and hence cultured in a specilized medium (TM, #7121, ScienCell Research Laboratories). RNA-seq analysis were performed using a illumina HiSeq2500 platform.