Dataset on the effect of Dendrobium officinale dietary fiber on the gut microbiome in high-fat-diet-induced obese mice

Obesity and related metabolic syndromes are becoming a major public
health problem due to excess dietary fat intake. Dendrobium officinale is a medicine food homology herb ranked as the
head of “nine kinds of precious medicinal herbs” in China and exerts multiple
health-promoting effects. In this study, mice
were fed with standard chow diet (Chow), high-fat diet (HFD) or HFD plus daily
administration of D. officinale dietary fiber (DODF, 1.0 g/kg) by
intragastric gavage for 11 weeks. To examine the gut microbial changes,
a 16S rRNA gene sequencing approach was used to analyze the gut microbiome in fecal
samples of mice fed with Chow, HFD and DODF. To investigate the impact of DODF on
host metabolism, a 1H NMR-based metabolomics approach was applied to
analyze metabolic changes in feces, serum, and liver. In addition, to elucidate
whether the gut flora acts as a causal factor for mediating the efficacies
of DODF, we next transferred the faecal microbiota
either from DODF-treated or vehicle-treated HFD-fed donor mice (DODF-R and
HFD-R) to HFD-induced obese mice for 14 days.