Three other human glioma cells make increased mRNA of EZH2, Whsc2 and YKL-40 under hypoxic conditions.

The human glioma cells (LNZ308, T98G and U87) were cultured at 1 million cells in 15 mls of media within T75 cm2 flasks either under hypoxic or normoxic conditions for 7 days. Afterwards, the mRNA was isolated and qRT-PCR was accomplished using the EZH2, Whsc2 and YKL-40 primers. Data is displayed as increased fold-differences of the hypoxic cells relative to the normoxic cells. The data represents the composite of three separate experiments and is presented as mean and the standard error of the means.