Proteomics and Bioinformatics Identify Drug-Resistant-Related Genes with Prognostic Potential in Cholangiocarcinoma

Resistance to commonly used chemotherapeutic drugs is a major problem of cholangiocarcinoma (CCA) therapy, therefore more effective treatment strategy is urgently needed. Drug resistant cell lines have been used for elucidating drug resistance mechanisms in various cancers leading to the discovery of novel treatments. Here in, we established a 5-Fluorouracil and Gemcitabine resistant CCA cell lines. The resistant sublines were selected based on constant exposure of KKU-213B parental cells to 5-Fluorouracil and gemcitabine in a stepwise dose incremental strategy starting from below IC25 concentration). The cells that grown normally in the presence of 5-FU 7 and Gem 3 μM were used for subsequence experiments and weredesignated as KKU-213B-FR and KKU-213B-GR. The resultant cell lines were confirmed to be resistant to chemotherapeutic drug and also increased cell migration and invasion activity. In addition, the quantitative LC-MS/MS were utilized for the analysis of proteins involved in drug resistance mechanisms.