Quantitative characteristics of EM48-positive objects in young and old rats.

Rats received intrastriatal injection of lentiviral vectors encoding the Htt171-82Q at different ages (young, 3 weeks; old, 15 months). Histological evaluation using EM48 immunohistochemistry for inclusions and neuropil aggregate detection was performed at 4 weeks post-infection. EM48-positive objects (inclusions and aggregates) were counted using an automated acquisition and image analysis system. Note that in older rats there is a reduction in the cross-sectional area of EM48-positive objects and a trend towards an increased number of objects.