Molecular and cellular signature of perirenal adipose tissue

We investigated whether the features of donor-derived stromal vascular fraction of perirenal adipose tissue (PRAT-SVF) could be indicative of the deleterious impact of the ECD microenvironment on renal transplants. A comparative analysis of cellular components, transcriptomic and vasculogenic profiles was performed in PRAT-SVF obtained from deceased donors (non ECD) and expanded criteria donors (ECD) The global RNA sequencing approach indicated a differential molecular signature in the PRAT-SVF of ECD donors characterized by the overexpression of inflammatory transcripts (CXCL1, CCL4, IL1-β,INF-ϒ). stromal vascular fraction mRNA profiles of non-ECD and ECD donors were generated using Illumina Nextseq sequencer.