DNA damage signaling in Drosophila macrophages modulates systemic cytokine levels in response to oxidative stress

Environmental factors, infection, or injury can cause oxidative stress in diverse tissues and loss of tissue homeostasis. Effective stress response cascades, conserved from invertebrates to mammals, ensure reestablishment of homeostasis and tissue repair. Hemocytes, the Drosophila blood-like cells, rapidly respond to oxidative stress by immune activation. However, the precise signals how they sense oxidative stress and integrate these signals to modulate and balance the response to oxidative stress in the adult fly are ill-defined. Furthermore, hemocyte diversification was not explored yet on oxidative stress. Here, we employed high throughput single nuclei RNA-sequencing to explore hemocytes and other cell types, such as fat body, during oxidative stress in the adult fly. We identified distinct cellular responder states in plasmatocytes, the Drosophila macrophages, associated with immune response and metabolic activation upon oxidative stress. We further define oxidative stress-induced..., Drosophila melanogaster stocks:
Flies were reared on a high yeast food containing 10% brewer’s yeast, 8% fructose, 2% polenta and 0.8% Agar. Propionic acid and nipagin were added to prevent bacterial or fungal growth. All crosses (except those containing the tub-Gal80ts construct or otherwise noted) were performed at 25°C with a 12 hours dark/light cycle. The crosses with tub-Gal80ts were performed at 18°C to ensure the inhibition of the Gal4-protein during developmental stages of the fly. The experimental male F1 flies were transferred to 29°C as soon as they hatched and were aged for six days. All transgenic lines used in this study are listed below:

List of Drosophila lines used in this study
Source

w1118;Hml∆ -Gal4,UAS-2xeGFP
Gift from Marc S. Dionne

w1118;UAS-tefu-IR
Vienna Drosophila Research Center

w1118;UAS-mei41-IR
Vienna Drosophila Research Center

w1118;UAS-nbs-IR
Vienna Drosophila Research Center

w1118;UAS-mei41-IR;UAS-tefu-IR
made for this study

w1118;UAS-..., , # Drosophila macrophage response to oxidative stress

The data deposited here includes survival data, imaging analysis, glucose/glycogen measurements, qPCR assays as well as TLC to measure triglyerides. The precise methods and analyses are described in the method section of this dataset.

## Description of the data and file structure

The data files deposited here include the following datasets:

Fly survivals were performed as indicated in the method section. Genotypes and treatment are included in the raw counting files below. Dead flies are indicated as 1 in the table living flies are indicated as 0.

*Survival data (Lifespan, Paraquat, Starvation)*

Paraquat survival:

Fig.1_S1A_PQ survival_2mM_15mM_30mM_raw data.xml:

Fig.1I_crq-reaper 15mM PQ survival raw data.txt

Fig.5_S1E_Hml;tubGal80_DDR-IR_survival15mMPQ_raw data.xml

Fig.5A_15mM PQ survival Hml-Gal4_DDR-IR.txt

Fig.6A_Hml-upd3 and Hml-upd3-IR 15mM PQ survival_raw data.xml

Fig.6C_Hml-upd3 and Hml-upd3-IR 15mM PQ hemocyte q...