16S rRNA gene sequencing of the chicken eggshell and intestinal tract

The objective of this study was to determine the impact of microbial communities on the eggshell and in the environment on the establishment of and successional processes in the chicken intestinal tract. The V4 region of the 16S rRNA gene was amplified to determine community composition of birds exposed to only eggshell microbiota, only environmental microbiota, and birds exposed to both.