ADDOMEx Tier 3 Experiments: Mesocosm #3 Gulf of Mexico open ocean waters (GOMOO) and Mesocosm #4 Gulf of Mexico coastal waters (GOMCOAST) 10 um filter size

To determine the effects of oil and dispersant on eukaryotic microbial communities, mesocosm experiments were run with natural microbial communities from both an open ocean and coastal site. Samples were taken every 12 hours and filtered onto 10um pore size filters to determine the large size fraction of the microbial community. Samples were then extracted for DNA, amplified using Bradley et al., 2016 primers for the 18S rRNA V8/V9 region and submitted for sequencing. Data include the raw paired-end reads from this analysis and was later processed using the bioinformatics tool, mothur. Data were combined with the 0.2um size fraction for whole community analysis.