Spatial RNA sequencing of regenerating mouse hindlimb muscle. Spatial RNA sequencing of regenerating mouse hindlimb muscle

We report a series of spatial transcriptomics datasets of regenerating mouse muscle tissue generated with the 10x Genomics Visium platform. Overall design: Tibialis anterior muscles were injected with notexin and collected two, five, and seven days post-injury. Samples were frozen in OCT. 10 micron tissue sections were assayed using the Visium Spatial Gene Expression Kit (v1). To improve resolution, each spot within the Visium slide was deconvolved using a matched single-cell RNA sequencing reference and BayesPrism (https://github.com/Danko-Lab/TED). Cell type fractions for each spot are included in the metadata files.