A study of Identification and verification of the role of key metabolites and metabolic pathways on ASFV replication, Zunji shi et.al

African swine fever (ASF), one of the most harmful diseases prevalent in pig farms nowadays, caused by the African swine fever virus (ASFV) that cause enormous economic losses. Viremia usually develops within a few days after ASFV infection. However, the metabolic changes in pig serum after ASFV infection remain unclear. In this study, serum samples collected from ASFV-infected pig at different times were analyzed using pseudotargeted metabolomics method. Metabolomic analysis revealed 225 metabolites decreased and 65 metabolites increased in serum collected in ASFV infected 5 days post-infection (dpi), 254 metabolites decreased and 58 metabolites increased in serum collected ASFV infected 10 dpi. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the significant changed metabolites indicated that dopaminergic synapse has the highest rich factor in both ASFV5 (ASFV infected 5 dpi) VS ASFV0 (ASFV infected 0 dpi) and ASFV10 (ASFV infected 10 dpi) VS ASFV0.