Bioinformatic and Computational Tools Developed in the ESBL

The Epithelial Systems Biology Laboratory (DIR,NHLBI,NIH), headed by Mark Knepper, has developed a number of computational tools for analysis of proteomic and DNA sequencing data and have made them available to the community. The .zip file contains web architecture for access to "Bioinformatic and Computational Tools". Full documentation is included on the various pages. The various links allow users to access online versions of the tools or download source code and/or executables. Please see https://esbl.nhlbi.nih.gov/GH-web-computational-tools/ for current implementation as of March 29, 2026. The following elements are included: AbDesigner - a tool for choosing peptide sequences from specific proteins that are immunogenic, unique, and lacking in post-translational modifications. Automated Bioinformatics Extractor (ABE) - a batch tool for extraction of specified information about specific proteins from UniProt or RefSeq protein records. Downloadable Tool for Modeling of Salt, Urea and Water Transport in a Renal Tubule Segment - Differential equation-based modeling tool. Kinase Predictor - predicts protein kinases most likely to phosphorylate a given site represented by an input amino acid sequence. Kinase Substrate Preference Logos - Interactive Table - substrate preference logos of mammalian protein kinases. Kinase Substrate Preference Logos - Phylogenetic Tree - interactive phylogenetic tree of mammalian protein kinases and their associated substrate preference logos. NGS-Integrator - a tool for combining multiple NGS data tracks into a single track using Bayesian methods. PhosphoLogo - a tool for generating information-based sequence logos from a list of equal-length peptide sequences surrounding a phosphorylated residue. ProMatch - a program to match peptide and mRNA sequences to entries in NCBI Reference Sequence Database (RefSeq). PTM Centralizer - converts MS-identified peptides containing a specific feature (e.g. a phosphorylation site) to a sequence of given length with the feature in the center. PTMLogo - an improved tool for generating information-based sequence logos from a list of equal-length peptide sequences surrounding a phosphorylated residue (Uses position-specific background). Renal Inner Medulla Free-Energy Calculator - a tool for calculation of net free energy change in steady-state models of the renal concentrating mechanism. Temporal Pattern Mining (TPM)algorithm - clusters time-series data sets according to time-course pattern. virtualBlot - a program for predicting location of bands on immunoblots based on protein mass spectrometry data from sliced SDS-PAGE gels. Virtual Western Blots of 8600 mouse proteins in mpkCCD cells.