Postnatal SETD1B is essential for learning and the regulation of neuronal plasticity genes. Postnatal SETD1B is essential for learning and the regulation of neuronal plasticity genes

We have analyzed changes in histone 3 lysine 4 methylation (H3K4me3 and H3K4me1), histone acetylation (H3K9ac and H3K27ac) and gene expression resulting from Setd1b knockdown in adult hippocampal CA neurons in mice. We implemented Cre/loxP-mediated conditional knockout strategy in order to knockdown this histone methyltransferase in excitatory forebrain neurons in adult mice. Overall design: Examination of H3K4me3, H3K4me1, H3K9ac and H3K27ac levels, and gene expression in hippocampal CA neurons of adult Setd1b cKO mice.