Wastewater dataset on the SARS-CoV-2 sublineages circulating in Central Arkansas, USA, post-COVID-19 pandemic

Data File 1. NCBI Sequence Read Archive accession numbers and metadata of wastewater samples collected in four wastewater treatment facilities in Central Arkansas, USA (Little Rock’s Adams Field, Fourche Creek, and Little Maumelle and Pine Bluff) between 08 February 2022 and 16 April 2024.
Data File 2. Mean Ct values of SARS-CoV-2 genes (Orf1ab, S-protein, and N-protein) and endogenous control (PMMoV) in wastewater samples collected in four wastewater treatment facilities in Central Arkansas, USA (Little Rock’s Adams Field, Fourche Creek, and Little Maumelle and Pine Bluff) between 08 February 2022 and 16 April 2024. Undetermined values were Ct > 40.
Data File 3. Lineage analysis of SARS-CoV-2 and consensus sequences (FASTA format) detected through RNA sequencing in wastewater samples collected in four wastewater treatment facilities in Central Arkansas, USA (Little Rock’s Adams Field, Fourche Creek, and Little Maumelle and Pine Bluff) between 08 February 2022 and 19 March 2024. The data were generated using Illumina DRAGEN COVID Lineage software, and the most abundant variants detected in each sample are shown. This software aligns SARS-CoV-2 sequence reads to a reference genome to generate a consensus genome sequence and identify mutations, including amino acid substitutions and deletions. It then performs lineage/clade classification using Pangolin and/or NextClade. aWorld Health Organization’s terminology used to classify SARS-CoV-2 variants. bPercentage of base calls in the consensus SARS-CoV-2 sequence that are not ambiguous, i.e., the software could confidently assign a specific nucleotide (A, T, C, or G).
Data File 4. Relative abundance of SARS-CoV-2 lineages based on amino acid changes in wastewater samples in Central Arkansas (Little Rock’s Adams Field, Fourche Creek, and Little Maumelle and Pine Bluff) from 02 November 2021 to 19 March 2024. The data was obtained by using the bioinformatic package Freyja (https://github.com/andersen-lab/Freyja)13.