Early metazoan cell type diversity and the evolution of multicellular gene regulation

We performed single-cell transcriptome analysis (using MARS-seq) in the sponge Amphimedon queenslandica (adult and larval stages), in the ctenophore Mnemiopsis leidyi (adult stage), and in the placozoan Trichoplax adhaerens (adult stage). Additionally we performed bulk iChIP experiments (2 replicates of each experiment) using antibodies against H3K4me3 and H3K4me2 in adult Mnemiopsis leidyi and adult Trichoplax adhaerens. Multiple animals (20-25) were dissociated and pooled before sorting single-cells for scRNAseq. 200,000 cells were used in bulk iChIP experiments.