Supporting data for "A high-quality genome assembly of the endangered golden snub-nosed monkey Rhinopithecus roxellana"

The golden snub-nosed monkey (Rhinopithecus roxellana), is an endangered colobine monkey species endemic to China. This species has several distinctive traits, and it is an ideal model for analysing evolutionary development of the social structure due to its unique social organization. Although there has been reported a genome assembly of the subspecies R. roxellana hubeiensis, the assembly is incomplete and fragmented due to employing short reads sequencing technology. This drawback may lose information, such as structural variation and repeat sequences which are important for understanding this endangered species. Therefore, to have a better understanding of evolutionary history and genetic-specific signatures, a high-quality reference genome of the taxon is need. To obtain a high-quality chromosome assembly of R. roxellana qinlingensis, we combined a total of five techniques including Pacific Bioscience’s single-molecule real-time sequencing, Illumina’s paired-end sequencing, BioNano optical maps, 10X Genomics link-reads and high-throughput chromosome conformation capture. The results indicate the assembled genome is about 3.04 Gb with a contig N50 of 5.72 Mbp and a scaffold N50 of 144.56 Mbp, which have made a 10-fold improvement compared to past published. It is shown that a total of 22497 protein coding genes were predicted, of which 22053 were functionally annotated. Moreover, gene family analysis shows that 993 and 2745 gene families are expanded and contracted in the R. roxellana genome, respectively. We present the updated high-quality genome assembly of R. roxellana with superior continuity and accuracy. The assembled genome can be used as reference for future genetic studies of the species. Also, the updated genome assembly may contribute to our comprehensive understanding of the species, which is particularly helpful in the conservation of this endangered species. Furthermore, such genome with superior continuity and accuracy can provide a new standard reference for Colobine primates.