Quantification of isoforms expressed in the venom samples.

MSFragger was set up to search a reverse concatenated in-house database consisting of the protein sequences predicted from a non-redundant master set containing venom toxins annotated from the transcriptome of venom glands from SB831, SB833, SB834, and SB1130 specimens, assuming the digestion by trypsin. The Normalized Total Spectra Counting (NTSC) was used to estimate the abundance of independent proteins and toxin groups. (S2_Table.XLSX)