RNA-seq of UGP2 mutant human embryonic stem cells and in vitro differentiated neural stem cells

We report transcriptome analysis of human embryonic stem cells and in vitro differentiated neural stem cells, comparing wild type H9, UGP2 homozygous knock-out and UGP2 mutant cells harboring a homozygous A>G nucleotide change affecting the start codon of UGP2 isoform 2 that was introduced by CRISPR-Cas9 engineering Overall design: For the RNA-seq experiments, two independent H9 wild type cultures, two independent knock-out clones harboring the same homozygous genetic alteration in UGP2 and two independent clones harboring the homozygous UGP2 mutation were used. All 6 cell lines (two biological replicates per genotype) were cultured in two technical replicates, and sequenced at the hESC state and as NSCs cultured at passage 5 upon NSC differentiation.