SYK phosphorylates PLCG2 in p-6Y-SYK:p-Y65,Y76-FCER1G:PLCG2

Activation of SYK triggers multiple cascades, which induces NF-kB activation through a CARD9-dependent pathway. Phospholipase C-gamma 2 (PLCG2) is one of the key signaling components of the CLEC4E (Mincle)/CLEC6A (Dectin-2) pathway that connects SYK activation to CARD9 recruitment. PLCG2 is activated upon CLEC4E (Mincle)/CLEC6A (Dectin-2) engagement and triggers an intracellular Ca2+ flux. SYK and Src family kinases are upstream of PLCG2. SYK phosphorylates PLCG2 on Y753 and Y759, enhancing the activity of PLCG2 (Gorjestani et al. 2011, Suzuki-Inoue et al. 2004).

SYK激酶的激活触发了多重级联反应，通过CARD9依赖性途径诱导NF-kB的激活。磷脂酶C-γ2（PLCG2）是连接SYK激活与CARD9募集的CLEC4E（Mincle）/CLEC6A（Dectin-2）途径中的关键信号传导组分之一。PLCG2在CLEC4E（Mincle）/CLEC6A（Dectin-2）的结合后被激活，并引发细胞内Ca2+的流动。SYK和Src家族激酶位于PLCG2的上游。SYK在Y753和Y759位点磷酸化PLCG2，从而增强PLCG2的活性（Gorjestani等，2011年；Suzuki-Inoue等，2004年）