Study of the vaginal microbiome during placebo-controlled oral yeast supplementation

In this study the vaginal microbiome was investigated by 16S amplicon sequencing of the V4 region using Illumina Miseq technology. 52 women were included and split over 3 groups: a placebo group (n=18) and two probiotic groups (n=17 each). The women in the probiotic groups received daily oral supplementation with either 500mg or 1000mg Saccharomyces cerevisiae CNCM I-3856 over a four week period. Vaginal samples were collected at 6 timepoints at specific points in the menstrual cycle: before intervention- after menstruation (V1), after 7 days of supplementation (D7), after 14 days of supplementation (D14), after 21 days of supplementation (D21), at the end of the intervention period and after menstruation (V2) and finally 2 weeks after the end of the supplementation period (V3).