Transcriptomic study revealing the effect of thiamin on the morphology of Yarrowia lipolytica

A comparison between Y. lipolytica growth in a Synthetic agar Medium not containing THiamin (SM TH-) to its growth in a Synthetic agar Medium supplemented with THiamin (SMTH+) at day 8 of the culture. This study allowed us to discover the effect of thiamin on the growth and the morphology of Y. lipolytica The transcriptomic study was carried out using Agilent 8X15k slides with a custom oligoset designed by V. Loux using the Yarrowia lipolytica ORFeome extracted from genolevures database Two conditions experiment: growth of Y. lipolytica in SM TH- versus growth of Y. lipolytica in SM TH+ at day 8 of culture. Dye switches were done using four biological replicates for both conditions.