PRMT1 differentially regulates gene expression in T47D-breast cancer cells

Using a genome-wide analysis, we demonstrated that PRMT1 is selectively required for progesterone-regulated expression of PR target genes, implicated in cell proliferation and migration. Overall design: We examined whether PRMT1 is required for progesterone-induced expression of genes, by depleting PRMT1 in T47D breast cancer cells, using siRNA. We examined gene expression changes at 6 hours progesterone (R5020) treatment compared with ethanol treatment as a control. siRNA for a nonspecific sequence (siCT) followed by 6h of R5020 treatment was performed as control.