Molecular analysis of platinum-resistant and -sensitive ovarian cacner based on RNA-Seq analysis

Ovarian cancer (OC) is the leading cause of death from gynecologic malignancies. The most difficult issue in the treatment of ovarian cancer is the eventual development of platinum resistance. Accumulating studies have shown that circRNAs are abnormally aberrantly expressed in tumors and play critical roles in tumor growth, metastasis, stemness and resistance to therapy.To identify circRNAs that play crucial roles in maintaining the platinum resistance of ovarian cacner, we performed RNA-seq analysis in platinum-resistant(n=9) and -sensitive(n=10) ovarian cacner tissues. Candidate genes were identified by bioinformatic analysis and literature review. Overall design: Tumor tissues from platinum-sensitive (n = 10) or platinum-resistance ( n = 9) ovarian cancer patients were prospectively collected before platinum-based therapies and analyzed by RNA-Seq on Illumina NovaSeq 6000 platform. Potential expression profile were compared between platinum-sensitive and platinum-resistance groups. Candidate genes were identified by bioinformatic analysis and literature review.