M. tuberculosis H37Rv: mutant expressing foreign peptide SL3 Vs control H37Rv
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M. tuberculosis H37Rv: mutant expressing foreign peptide SL3 Vs control H37Rv
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2014-04-10
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Strains and Plasmids.
aHyg = hygromycin, Tet = tetracycline, oriM = mycobacterial pAL5000 origin of replication [47], [62], Phsp60 = promoter from 60 kDa heat shock protein [63], PL5 = promoter from the L5 mycoba
NIAID Data Ecosystem40
The Development of a Novel Mycobacterium-Escherichia coli Shuttle Vector System Using pMyong2, a Linear Plasmid from Mycobacterium yongonense DSM 45126T
The Mycobacterium-Escherichia coli shuttle vector system, equipped with the pAL5000 replicon, is widely used for heterologous gene expression and gene delivery in mycobacteria. Despite its extensive u
Figshare2016-01-15 更新20
The Development of a Novel Mycobacterium-Escherichia coli Shuttle Vector System Using pMyong2, a Linear Plasmid from Mycobacterium yongonense DSM 45126 T
The Mycobacterium-Escherichia coli shuttle vector system, equipped with the pAL5000 replicon, is widely used for heterologous gene expression and gene delivery in mycobacteria. Despite its extensive u
NIAID Data Ecosystem10
Sequencing of the insert of the final construct used to generate the ∆ rv1877 M.tb mutant
Here, the fragment of pGOAL17 containing the sacB and LacZ has been cloned into the p2NIL-1877US/DS construct, and this was sequenced again to check for integrity. Since the insert is la
Figshare2024-02-16 更新30
Comparative electrotransformation efficiency and spontaneous resistance to different antibiotics in M. chelonae strains ATCC 35752 and 9917, and M. abscessus ATCC 19977.
Transformation efficiencies upon selection with the indicated antibiotics on 7H11-OADC agar are expressed as numbers of drug-resistant CFUs per µg of DNA electroporated. The percentage below each tran
Figshare2015-12-02 更新10
