Metaproteomics analysis of the thiocyanate-degrading microflora SCN- degradation

Screening of domesticated complex flora from wastewater can degrade high concentrations of thiocyanate, the molecular mechanism of this phenomenon remains unclear. In this study, macro-proteomics techniques were used to analyse the intracellular proteins of the composite flora. The researchers performed proteomic analysis using combined tandem mass spectrometry to identify the differential proteins of the colonies under glucose treatment and thiocyanate stress. A total of 4,747 differential proteins were detected across the three different periods. Of these, 1,213 differential proteins were detected in the pre-biodegradation period, 1,300 differential proteins in the mid-biodegradation period, and 1,413 differential proteins in the post-biodegradation period. Through the protein function annotation, it was found that the differential proteins were mainly concentrated in carbon metabolism, energy metabolism, two-component system and amino acid metabolism.