Quantification of tRNA-derived fragments in Arabidopsis and rice

Here, we adopt a method that combines tRNA-seq and cp-RNA-seq to identify and quantify tRFs and tRNAs in plants. We provide a high-quality expression atlas of tRFs and tRNAs in Arabidopsis and rice, and uncovers complex tRFs repertoire and the dynamic expressions of tRNA genes in plants. We constructed five Arabidopsis tRNA-seq libraries sampling from various vegetative and reproductive tissues, and seven rice libraries from shoots, roots and panicles. As abiotic stresses potentially induce tRFs levels, the rice seedlings with salt and cold treatments were also subjected to tRNA-seqs. Two biological replicates for each sample were performed.